› 2p6r

X-ray diffraction
3Å resolution

Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA

Released:
DOI: 10.2210/pdb2p6r/pdb
PDB entry 2p6r coloured by chain, front view.
PDB entry 2p6r coloured by chain, side view.
PDB entry 2p6r coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Structural basis for DNA duplex separation by a superfamily-2 helicase.
Büttner K, Nehring S, Hopfner KP
Nat Struct Mol Biol 14 647-52 (2007)
PMID: 17558417

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-115755 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
afUHEL308 HELICASE Chain: A
Molecule details ›
Chain: A
Length: 702 amino acids
Theoretical weight: 79.12 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
Structure domains:
25-MER Chain: X
Molecule details ›
Chain: X
Length: 25 nucleotides
Theoretical weight: 7.66 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided
5'-D(*CP*TP*AP*GP*AP*GP*AP*CP*TP*AP*TP*CP*GP*AP*T)-3' Chain: Y
Molecule details ›
Chain: Y
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6122
Unit cell:
a: 138.379Å b: 138.379Å c: 252.973Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.232 0.275
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

47 review citations

Translocation and unwinding mechanisms of RNA and DNA helicases.
Pyle AM. (2008)
46 more

50 mentions without citation

XPB and XPD helicases in TFIIH orchestrate DNA duplex opening and damage verification to coordinate repair with transcription and cell cycle via CAK kinase.
Fuss et al. (2011)
49 more