› 2p1r

X-ray diffraction
2.5Å resolution

Crystal structure of Salmonella typhimurium YegS, a putative lipid kinase homologous to eukaryotic sphingosine and diacylglycerol kinases.

Released:
DOI: 10.2210/pdb2p1r/pdb
PDB entry 2p1r coloured by chain, front view.
PDB entry 2p1r coloured by chain, side view.
PDB entry 2p1r coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Characterization of Salmonella typhimurium YegS, a putative lipid kinase homologous to eukaryotic sphingosine and diacylglycerol kinases.
Nichols CE, Lamb HK, Lockyer M, Charles IG, Pyne S, Hawkins AR, Stammers DK
Proteins 68 13-25 (2007)
PMID: 17393457

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187308 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable lipid kinase YegS Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 299 amino acids
Theoretical weight: 32.39 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZNP1 (Residues: 1-299; Coverage: 100%)
Gene names: STM2140, yegS
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 2 x NA
Ligand CA 10 x CA
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 107Å b: 70.99Å c: 102.16Å
α: 90° β: 118.51° γ: 90°
R-values:
R R work R free
0.206 0.206 0.255
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Regulation of sphingosine kinase and sphingolipid signaling.
Pitson SM. (2011)
3 more

1 mention without citation

Dynamic features of homodimer interfaces calculated by normal-mode analysis.
Tsuchiya et al. (2012)