› 2oxf

X-ray diffraction
1.76Å resolution

X-ray structure of the unliganded uridine phosphorylase from Salmonella typhimurium in homodimeric form at 1.76A resolution

Released:
DOI: 10.2210/pdb2oxf/pdb
PDB entry 2oxf coloured by chain, front view.
PDB entry 2oxf coloured by chain, side view.
PDB entry 2oxf coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Entry authors: Timofeev VI, Pavlyk BP, Lashkov AA, Gabdoulkhakov AG, Mikhailov AM

Function and Biology Details

Reaction catalysed: 
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, F
Molecule details ›
Chains: A, F
Length: 250 amino acids
Theoretical weight: 26.82 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A1F6 (Residues: 4-253; Coverage: 99%)
Gene names: STM3968, STMD1.21, udp
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: R3
Unit cell:
a: 150.86Å b: 150.86Å c: 47.84Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.163 0.207
Expression system: Escherichia coli BL21(DE3)

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