› 2owz

X-ray diffraction
2.18Å resolution

R-state, citrate and Fru-6-P-bound Escherichia coli fructose-1,6-bisphosphatase

Released:
DOI: 10.2210/pdb2owz/pdb
PDB entry 2owz coloured by chain, front view.
PDB entry 2owz coloured by chain, side view.
PDB entry 2owz coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structures of activated fructose-1,6-bisphosphatase from Escherichia coli. Coordinate regulation of bacterial metabolism and the conservation of the R-state.
Hines JK, Fromm HJ, Honzatko RB
J Biol Chem 282 11696-704 (2007)
PMID: 17314096

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141852 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-1,6-bisphosphatase class 1 Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 37.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A993 (Residues: 1-332; Coverage: 100%)
Gene names: JW4191, b4232, fbp, fdp
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand F6P 1 x F6P
Ligand CIT 1 x CIT
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I222
Unit cell:
a: 45.315Å b: 83.332Å c: 173.415Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.208 0.249
Expression system: Escherichia coli

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Citations

1 review citation

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
14 other articles

2 mentions without citation

Structural, kinetic and proteomic characterization of acetyl phosphate-dependent bacterial protein acetylation.
Kuhn et al. (2014)
1 more