› 2orw

X-ray diffraction
1.5Å resolution

Thermotoga maritima thymidine kinase 1 like enzyme in complex with TP4A

Released:
DOI: 10.2210/pdb2orw/pdb
PDB entry 2orw coloured by chain, front view.
PDB entry 2orw coloured by chain, side view.
PDB entry 2orw coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Binding of ATP to TK1-like enzymes is associated with a conformational change in the quaternary structure.
Segura-Peña D, Lutz S, Monnerjahn C, Konrad M, Lavie A
J Mol Biol 369 129-41 (2007)
PMID: 17407781

Function and Biology Details

Reaction catalysed: 
ATP + thymidine = ADP + thymidine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194522 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 20.68 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYN2 (Residues: 1-184; Coverage: 100%)
Gene names: TM_0401, tdk
Sequence domains: Thymidine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 2 x MG
Ligand 4TA 2 x 4TA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2
Unit cell:
a: 102.55Å b: 59.63Å c: 61.44Å
α: 90° β: 103.11° γ: 90°
R-values:
R R work R free
0.173 0.169 0.208
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Thymidine kinase 1 through the ages: a comprehensive review.
Bitter et al. (2020)
2 more

5 mentions without citation

Prediction of structures of zinc-binding proteins through explicit modeling of metal coordination geometry.
Wang et al. (2010)
4 more