› 2ong

X-ray diffraction
2.7Å resolution

Crystal Structure of of limonene synthase with 2-fluorogeranyl diphosphate (FGPP).

Released:
DOI: 10.2210/pdb2ong/pdb
PDB entry 2ong coloured by chain, front view.
PDB entry 2ong coloured by chain, side view.
PDB entry 2ong coloured by chain, top view.
Source organism: Mentha spicata
Primary publication:
Structure of limonene synthase, a simple model for terpenoid cyclase catalysis.
Hyatt DC, Youn B, Zhao Y, Santhamma B, Coates RM, Croteau RB, Kang C
Proc Natl Acad Sci U S A 104 5360-5 (2007)
PMID: 17372193

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4S-limonene synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 543 amino acids
Theoretical weight: 63.9 KDa
Source organism: Mentha spicata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q40322 (Residues: 57-599; Coverage: 91%)
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 6 x MN
Ligand FPG 2 x FPG
Ligand BTB 4 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: I4
Unit cell:
a: 200.48Å b: 200.48Å c: 123.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.241
Expression system: Escherichia coli

Quick links

Citations

19 review citations

The family of terpene synthases in plants: a mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom.
Chen et al. (2011)
18 more

20 mentions without citation

Terpenoid synthase structures: a so far incomplete view of complex catalysis.
Gao et al. (2012)
19 more