› 2olq

X-ray diffraction
1.94Å resolution

How Does an Enzyme Recognize CO2?

Released:
DOI: 10.2210/pdb2olq/pdb
PDB entry 2olq coloured by chain, front view.
PDB entry 2olq coloured by chain, side view.
PDB entry 2olq coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
How does an enzyme recognize CO2?
Cotelesage JJ, Puttick J, Goldie H, Rajabi B, Novakovski B, Delbaere LT
Int J Biochem Cell Biol 39 1204-10 (2007)
PMID: 17475535

Function and Biology Details

Reaction catalysed: 
ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149561 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase (ATP) Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 59.71 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P22259 (Residues: 1-540; Coverage: 100%)
Gene names: JW3366, b3403, pck, pckA
Sequence domains: Phosphoenolpyruvate carboxykinase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MN 1 x MN
Ligand MG 1 x MG
Ligand ATP 1 x ATP
Ligand CO2 1 x CO2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: C2
Unit cell:
a: 125.584Å b: 91.369Å c: 46.577Å
α: 90° β: 96.22° γ: 90°
R-values:
R R work R free
0.204 0.205 0.235
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural insights into the mechanism of phosphoenolpyruvate carboxykinase catalysis.
Carlson et al. (2009)
1 more

3 mentions without citation

Using iterative fragment assembly and progressive sequence truncation to facilitate phasing and crystal structure determination of distantly related proteins.
Wang et al. (2016)
2 more