› 2o8n

X-ray diffraction
2Å resolution

Crystal Structure of Mouse Apolipoprotein A-I Binding Protein

Released:
DOI: 10.2210/pdb2o8n/pdb
PDB entry 2o8n coloured by chain, front view.
PDB entry 2o8n coloured by chain, side view.
PDB entry 2o8n coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Biochemical and structural characterization of apolipoprotein A-I binding protein, a novel phosphoprotein with a potential role in sperm capacitation.
Jha KN, Shumilin IA, Digilio LC, Chertihin O, Zheng H, Schmitz G, Visconti PE, Flickinger CJ, Minor W, Herr JC
Endocrinology 149 2108-20 (2008)
PMID: 18202122

Function and Biology Details

Reaction catalysed: 
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185092 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(P)H-hydrate epimerase Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8K4Z3 (Residues: 25-282; Coverage: 92%)
Gene names: Aibp, Apoa1bp, Naxe
Sequence domains: YjeF-related protein N-terminus
Structure domains: YjeF N-terminal domain

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: R32
Unit cell:
a: 124.312Å b: 124.312Å c: 120.677Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.169 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Phenotyping male infertility in the mouse: how to get the most out of a 'non-performer'.
Borg et al. (2010)
2 more

4 mentions without citation

Crystal structure of human Edc3 and its functional implications.
Ling et al. (2008)
3 more