2o8k

Solution NMR

NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element

Released:
Source organism: Aquifex aeolicus

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-115722 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
RNA polymerase sigma factor RpoN Chain: A
Molecule details ›
Chain: A
Length: 63 amino acids
Theoretical weight: 7.49 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66858 (Residues: 338-398; Coverage: 15%)
Gene names: aq_599, rpoN
Sequence domains: Sigma-54, DNA binding domain
Structure domains: Homeodomain-like
5'-D(*TP*TP*TP*TP*GP*GP*CP*AP*CP*GP*TP*TP*TP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.25 KDa
Source organism: Aquifex aeolicus
Expression system: Not provided
5'-D(*GP*AP*AP*AP*CP*GP*TP*GP*CP*CP*AP*AP*AP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.31 KDa
Source organism: Aquifex aeolicus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: docking, rigid-body minimization, simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided