› 2nuk

X-ray diffraction
1.2Å resolution

Soluble Domain of the Rieske Iron-Sulfur Protein from Rhodobacter sphaeroides

Released:
DOI: 10.2210/pdb2nuk/pdb
PDB entry 2nuk coloured by chain, front view.
PDB entry 2nuk coloured by chain, side view.
PDB entry 2nuk coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Atomic resolution structures of rieske iron-sulfur protein: role of hydrogen bonds in tuning the redox potential of iron-sulfur clusters.
Kolling DJ, Brunzelle JS, Lhee S, Crofts AR, Nair SK
Structure 15 29-38 (2007)
PMID: 17223530

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquinol-cytochrome c reductase iron-sulfur subunit Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 15.16 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02762 (Residues: 47-187; Coverage: 75%)
Gene names: fbcF, petA
Sequence domains: Rieske [2Fe-2S] domain
Structure domains: Rieske [2Fe-2S] iron-sulphur domain

Ligands and Environments

1 bound ligand:
Ligand FES 1 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I4
Unit cell:
a: 70.568Å b: 70.568Å c: 54.769Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.12 0.118 0.136
Expression system: Escherichia coli

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Citations

7 review citations

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
6 more

6 mentions without citation

The Q-cycle reviewed: How well does a monomeric mechanism of the bc(1) complex account for the function of a dimeric complex?
Crofts et al. (2008)
5 more