› 2nrp

X-ray diffraction
3Å resolution

MoeA R350A

Released:
DOI: 10.2210/pdb2nrp/pdb
PDB entry 2nrp coloured by chain, front view.
PDB entry 2nrp coloured by chain, side view.
PDB entry 2nrp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Mutational analysis of Escherichia coli MoeA: two functional activities map to the active site cleft.
Nichols JD, Xiang S, Schindelin H, Rajagopalan KV
Biochemistry 46 78-86 (2007)
PMID: 17198377

Function and Biology Details

Reaction catalysed: 
Adenylyl-molybdopterin + molybdate = molybdenum cofactor + AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146124 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Molybdopterin molybdenumtransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 411 amino acids
Theoretical weight: 44.02 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P12281 (Residues: 1-411; Coverage: 100%)
Gene names: JW0811, b0827, bisB, chlE, moeA, narE
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P212121
Unit cell:
a: 88.371Å b: 98.74Å c: 100.226Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.217 0.29
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Sulfite oxidizing enzymes.
Feng et al. (2007)
1 more