› 2nq7

X-ray diffraction
1.6Å resolution

Crystal structure of type 1 human methionine aminopeptidase in complex with 3-(2,2-Dimethylpropionylamino)pyridine-2-carboxylic acid thiazole-2-ylamide

Released:
DOI: 10.2210/pdb2nq7/pdb
PDB entry 2nq7 coloured by chain, front view.
PDB entry 2nq7 coloured by chain, side view.
PDB entry 2nq7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Elucidation of the function of type 1 human methionine aminopeptidase during cell cycle progression.
Hu X, Addlagatta A, Lu J, Matthews BW, Liu JO
Proc Natl Acad Sci U S A 103 18148-53 (2006)
PMID: 17114291

Function and Biology Details

Reaction catalysed: 
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine aminopeptidase 1 Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 36.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53582 (Residues: 81-384; Coverage: 79%)
Gene names: KIAA0094, METAP1
Sequence domains: Metallopeptidase family M24
Structure domains: Creatinase/methionine aminopeptidase superfamily

Ligands and Environments

4 bound ligands:
Ligand CO 3 x CO
Ligand K 1 x K
Ligand HM5 1 x HM5
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 47.317Å b: 77.442Å c: 47.221Å
α: 90° β: 92.31° γ: 90°
R-values:
R R work R free
0.195 0.195 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Metallo-aminopeptidase inhibitors.
Mucha et al. (2010)
6 more

3 mentions without citation

Elucidation of the function of type 1 human methionine aminopeptidase during cell cycle progression.
Hu et al. (2006)
2 more