› 2n5g

Solution NMR

NMR structure of KorA, a plasmid-encoded, global transcription regulator KorA

Released:
DOI: 10.2210/pdb2n5g/pdb
PDB entry 2n5g coloured by chain, ensemble of 20 models, front view.
PDB entry 2n5g coloured by chain, ensemble of 20 models, side view.
PDB entry 2n5g coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli
Primary publication:
Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator.
Rajasekar KV, Lovering AL, Dancea F, Scott DJ, Harris SA, Bingle LE, Roessle M, Thomas CM, Hyde EI, White SA
Nucleic Acids Res 44 4947-56 (2016)
PMID: 27016739

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TrfB transcriptional repressor protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 101 amino acids
Theoretical weight: 11.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03052 (Residues: 1-101; Coverage: 100%)
Gene names: korA, trfB
Sequence domains: TrfB plasmid transcriptional repressor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 41%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Intrinsic disorder in the partitioning protein KorB persists after co-operative complex formation with operator DNA and KorA.
Hyde et al. (2017)

3 mentions without citation

Unique Properties of the Alpha-Helical DNA-Binding Protein KfrA Encoded by the IncU Incompatibility Group Plasmid RA3 and Its Host-Dependent Role in Plasmid Maintenance.
Lewicka et al. (2021)
2 more