› 2mpw

Solution NMR

Solution structure of the LysM region of the E. coli Intimin periplasmic domain

Released:
DOI: 10.2210/pdb2mpw/pdb
PDB entry 2mpw coloured by chain, ensemble of 23 models, front view.
PDB entry 2mpw coloured by chain, ensemble of 23 models, side view.
PDB entry 2mpw coloured by chain, ensemble of 23 models, top view.
Source organism: Escherichia coli
Primary publication:
The Intimin periplasmic domain mediates dimerisation and binding to peptidoglycan.
Leo JC, Oberhettinger P, Chaubey M, Schütz M, Kühner D, Bertsche U, Schwarz H, Götz F, Autenrieth IB, Coles M, Linke D
Mol Microbiol 95 80-100 (2015)
PMID: 25353290

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148739 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Intimin Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 12.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P19809 (Residues: 39-143; Coverage: 11%)
Gene names: E2348C_3939, eae, eaeA
Sequence domains: LysM domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 90%
Refinement method: simulated annealing, NOESY back-calculation
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Type V Secretion Systems: An Overview of Passenger Domain Functions.
Meuskens et al. (2019)
7 more

4 mentions without citation

Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins.
Hatlem et al. (2019)
3 more