› 2map

Solution NMR

Solution structure of the complex formed by the region 2 of E. coli sigmaE and its cognate -10 promoter element non template strand TGTCAAA.

Released:
DOI: 10.2210/pdb2map/pdb
PDB entry 2map coloured by chain, ensemble of 18 models, front view.
PDB entry 2map coloured by chain, ensemble of 18 models, side view.
PDB entry 2map coloured by chain, ensemble of 18 models, top view.
Source organism: Escherichia coli DH1
Primary publication:
Structural basis for -10 promoter element melting by environmentally induced sigma factors.
Campagne S, Marsh ME, Capitani G, Vorholt JA, Allain FH
Nat Struct Mol Biol 21 269-76 (2014)
PMID: 24531660

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-115600 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
RNA polymerase sigma factor Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 11.02 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
Structure domains: Rna Polymerase Sigma Factor; Chain: A
DNA Chain: B
Molecule details ›
Chain: B
Length: 7 nucleotides
Theoretical weight: 2.12 KDa
Source organism: Escherichia coli DH1
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 75%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

Bacterial sigma factors: a historical, structural, and genomic perspective.
Feklístov et al. (2014)
15 more

1 mention without citation

Rewiring the specificity of extracytoplasmic function sigma factors.
Todor et al. (2020)