› 2ly5

Solution NMR

Refined solution structure of recombinant brazzein

Released:
DOI: 10.2210/pdb2ly5/pdb
PDB entry 2ly5 coloured by chain, ensemble of 15 models, front view.
PDB entry 2ly5 coloured by chain, ensemble of 15 models, side view.
PDB entry 2ly5 coloured by chain, ensemble of 15 models, top view.
Source organism: Pentadiplandra brazzeana
Primary publication:
Temperature-dependent conformational change affecting Tyr11 and sweetness loops of brazzein.
Cornilescu CC, Cornilescu G, Rao H, Porter SF, Tonelli M, DeRider ML, Markley JL, Assadi-Porter FM
Proteins 81 919-25 (2013)
PMID: 23349025

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Defensin-like protein Chain: A
Molecule details ›
Chain: A
Length: 53 amino acids
Theoretical weight: 6.38 KDa
Source organism: Pentadiplandra brazzeana
Expression system: Escherichia coli
UniProt:
  • Canonical: P56552 (Residues: 2-54; Coverage: 98%)
Structure domains: Knottin, scorpion toxin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 89%
Refinement method: molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Expression of a triple mutational des-pGlu brazzein in transgenic mouse milk.
Lu et al. (2022)
1 more

6 mentions without citation

Integrative NMR for biomolecular research.
Lee et al. (2016)
5 more