› 2ldb

X-ray diffraction
3Å resolution

STRUCTURE DETERMINATION AND REFINEMENT OF BACILLUS STEAROTHERMOPHILUS LACTATE DEHYDROGENASE

Released:
DOI: 10.2210/pdb2ldb/pdb
PDB entry 2ldb coloured by chain, front view.
PDB entry 2ldb coloured by chain, side view.
PDB entry 2ldb coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structure determination and refinement of Bacillus stearothermophilus lactate dehydrogenase.
Piontek K, Chakrabarti P, Schär HP, Rossmann MG, Zuber H
Proteins 7 74-92 (1990)
PMID: 2330370

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 317 amino acids
Theoretical weight: 34.9 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
UniProt:
  • Canonical: P00344 (Residues: 1-317; Coverage: 100%)
Gene names: lct, ldh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
2 bound ligands:
Ligand SO4 8 x SO4
Ligand FBP 2 x FBP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P61
Unit cell:
a: 86.8Å b: 86.8Å c: 356.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.26 not available not available
Expression system: Not provided

Quick links

Citations

1 review citation

The application of the molecular replacement method to the de novo determination of protein structure.
Lawrence MC. (1991)
29 other articles

4 mentions without citation

Development of quantitative structure-binding affinity relationship models based on novel geometrical chemical descriptors of the protein-ligand interfaces.
Zhang et al. (2006)
3 more