› 2lcp

Solution NMR

NMR structure of calcium loaded, un-myristoylated human NCS-1

Released:
DOI: 10.2210/pdb2lcp/pdb
PDB entry 2lcp coloured by chain, ensemble of 20 models, front view.
PDB entry 2lcp coloured by chain, ensemble of 20 models, side view.
PDB entry 2lcp coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
The C-terminal tail of human neuronal calcium sensor 1 regulates the conformational stability of the Ca²⁺₋ activated state.
Heidarsson PO, Bjerrum-Bohr IJ, Jensen GA, Pongs O, Finn BE, Poulsen FM, Kragelund BB
J Mol Biol 417 51-64 (2012)
PMID: 22227393

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuronal calcium sensor 1 Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62166 (Residues: 1-190; Coverage: 100%)
Gene names: FLUP, FREQ, NCS1
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 58%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Calcium Sensors in Neuronal Function and Dysfunction.
Burgoyne et al. (2019)
2 more

13 mentions without citation

The Complex Conformational Dynamics of Neuronal Calcium Sensor-1: A Single Molecule Perspective.
Choudhary et al. (2018)
12 more