Function and Biology Details
Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
Ubiquitin conjugation factor E4 B (preferred)
PDBe Complex ID:
PDB-CPX-131831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin conjugation factor E4 B
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: U-box domain
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Length: 100 amino acids
Theoretical weight: 11.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
O95155 (Residues: 1208-1302; Coverage: 7%)
Sequence domains: U-box domain
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Refinement method:
DISTANCE GEOMETRY, SIMULATED ANNEALING
Expression system: Escherichia coli
