› 2k31

Solution NMR

Solution Structure of cGMP-binding GAF domain of Phosphodiesterase 5

Released:
DOI: 10.2210/pdb2k31/pdb
PDB entry 2k31 coloured by chain, ensemble of 20 models, front view.
PDB entry 2k31 coloured by chain, ensemble of 20 models, side view.
PDB entry 2k31 coloured by chain, ensemble of 20 models, top view.
Source organism: Mus musculus
Primary publication:
Solution structure of the cGMP binding GAF domain from phosphodiesterase 5: insights into nucleotide specificity, dimerization, and cGMP-dependent conformational change.
Heikaus CC, Stout JR, Sekharan MR, Eakin CM, Rajagopal P, Brzovic PS, Beavo JA, Klevit RE
J Biol Chem 283 22749-59 (2008)
PMID: 18534985

Function and Biology Details

Reaction catalysed: 
Guanosine 3',5'-cyclic phosphate + H(2)O = guanosine 5'-phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183959 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-specific 3',5'-cyclic phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 176 amino acids
Theoretical weight: 19.97 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8CG03 (Residues: 153-320; Coverage: 19%)
Gene names: Pde5, Pde5a
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:
Ligand 35G 1 x 35G
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

6 review citations

cGMP-dependent protein kinases and cGMP phosphodiesterases in nitric oxide and cGMP action.
Francis et al. (2010)
5 more

7 mentions without citation

Cyclic nucleotide binding GAF domains from phosphodiesterases: structural and mechanistic insights.
Heikaus et al. (2009)
6 more