› 2k1g

Solution NMR

Solution NMR structure of lipoprotein spr from Escherichia coli K12. Northeast Structural Genomics target ER541-37-162

Released:
DOI: 10.2210/pdb2k1g/pdb
PDB entry 2k1g coloured by chain, ensemble of 20 models, front view.
PDB entry 2k1g coloured by chain, ensemble of 20 models, side view.
PDB entry 2k1g coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli K-12
Primary publication:
Solution NMR structure of the NlpC/P60 domain of lipoprotein Spr from Escherichia coli: structural evidence for a novel cysteine peptidase catalytic triad.
Aramini JM, Rossi P, Huang YJ, Zhao L, Jiang M, Maglaqui M, Xiao R, Locke J, Nair R, Rost B, Acton TB, Inouye M, Montelione GT
Biochemistry 47 9715-7 (2008)
PMID: 18715016

Function and Biology Details

Reaction catalysed: 
GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine + H(2)O = GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + D-alanine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Murein DD-endopeptidase MepS/Murein LD-carboxypeptidase Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.58 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFV4 (Residues: 63-188; Coverage: 78%)
Gene names: JW2163, b2175, mepS, spr, yeiV
Sequence domains: NlpC/P60 family
Structure domains: endopeptidase domain like (from Nostoc punctiforme)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

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Citations

4 review citations

Bacterial cell division regulation by Ser/Thr kinases: a structural perspective.
Ruggiero et al. (2012)
3 more

12 mentions without citation

Structure of a peptidoglycan amidase effector targeted to Gram-negative bacteria by the type VI secretion system.
Chou et al. (2012)
11 more