› 2jzo

Solution NMR

Solution NMR structure of the non-productive complex between IIAMannose and IIBMannose of the mannose transporter of the E. coli phosphotransferase system

Released:
DOI: 10.2210/pdb2jzo/pdb
PDB entry 2jzo coloured by chain, front view.
PDB entry 2jzo coloured by chain, side view.
PDB entry 2jzo coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Solution NMR structures of productive and non-productive complexes between the A and B domains of the cytoplasmic subunit of the mannose transporter of the Escherichia coli phosphotransferase system.
Hu J, Hu K, Williams DC, Komlosh ME, Cai M, Clore GM
J Biol Chem 283 11024-37 (2008)
PMID: 18270202

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + D-mannose(Side 1) = [protein]-L-histidine + D-mannose 6-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159666 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PTS system mannose-specific EIIAB component Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 14.45 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69797 (Residues: 2-134; Coverage: 41%)
Gene names: JW1806, b1817, gptB, manX, ptsL
Sequence domains: PTS system fructose IIA component
Structure domains: Phosphotransferase system, mannose-type IIA component
PTS system mannose-specific EIIAB component Chain: D
Molecule details ›
Chain: D
Length: 165 amino acids
Theoretical weight: 18.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69797 (Residues: 159-323; Coverage: 51%)
Gene names: JW1806, b1817, gptB, manX, ptsL
Sequence domains: PTS system sorbose subfamily IIB component
Structure domains: Phosphotransferase system, sorbose subfamily IIB component

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: conjoined rigid body/torsion angle simulated annealing
Expression system: Escherichia coli

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Citations

4 review citations

Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules and their complexes.
Clore et al. (2009)
3 more