› 2jql

Solution NMR

NMR structure of the yeast Dun1 FHA domain in complex with a doubly phosphorylated (pT) peptide derived from Rad53 SCD1

Released:
DOI: 10.2210/pdb2jql/pdb
PDB entry 2jql coloured by chain, ensemble of 20 models, front view.
PDB entry 2jql coloured by chain, ensemble of 20 models, side view.
PDB entry 2jql coloured by chain, ensemble of 20 models, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Diphosphothreonine-specific interaction between an SQ/TQ cluster and an FHA domain in the Rad53-Dun1 kinase cascade.
Lee H, Yuan C, Hammet A, Mahajan A, Chen ES, Wu MR, Su MI, Heierhorst J, Tsai MD
Mol Cell 30 767-78 (2008)
PMID: 18570878

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149543 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA damage response protein kinase DUN1 Chain: A
Molecule details ›
Chain: A
Length: 151 amino acids
Theoretical weight: 17.39 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39009 (Residues: 19-159; Coverage: 28%)
Gene names: D2370, DUN1, YDL101C
Sequence domains: FHA domain
Structure domains: Tumour Suppressor Smad4
Serine/threonine-protein kinase RAD53 Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.28 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P22216 (Residues: 3-12; Coverage: 1%)
Gene names: MEC2, P2588, RAD53, SAD1, SPK1, YPL153C

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Taking the time to make important decisions: the checkpoint effector kinases Chk1 and Chk2 and the DNA damage response.
Stracker et al. (2009)
4 more

4 mentions without citation

Specific recognition of a multiply phosphorylated motif in the DNA repair scaffold XRCC1 by the FHA domain of human PNK.
Ali et al. (2009)
3 more