› 2jf7

X-ray diffraction
2.48Å resolution

Structure of Strictosidine Glucosidase

Released:
DOI: 10.2210/pdb2jf7/pdb
PDB entry 2jf7 coloured by chain, front view.
PDB entry 2jf7 coloured by chain, side view.
PDB entry 2jf7 coloured by chain, top view.
Source organism: Rauvolfia serpentina
Primary publication:
Molecular architecture of strictosidine glucosidase: the gateway to the biosynthesis of the monoterpenoid indole alkaloid family.
Barleben L, Panjikar S, Ruppert M, Koepke J, Stöckigt J
Plant Cell 19 2886-97 (2007)
PMID: 17890378

Function and Biology Details

Reaction catalysed: 
Strictosidine + H(2)O = D-glucose + strictosidine aglycone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Strictosidine-O-beta-D-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 532 amino acids
Theoretical weight: 60.96 KDa
Source organism: Rauvolfia serpentina
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GU20 (Residues: 1-532; Coverage: 100%)
Gene name: SGR1
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P4212
Unit cell:
a: 157.63Å b: 157.63Å c: 103.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.239
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Alkaloid biosynthesis: metabolism and trafficking.
Ziegler et al. (2008)
10 more

1 mention without citation

A Phylogenetically Informed Comparison of GH1 Hydrolases between Arabidopsis and Rice Response to Stressors.
Cao et al. (2017)