› 2j3v

X-ray diffraction
2.11Å resolution

Crystal structure of the enzymatic component C2-I of the C2-toxin from Clostridium botulinum at pH 3.0

Released:
DOI: 10.2210/pdb2j3v/pdb
PDB entry 2j3v coloured by chain, front view.
PDB entry 2j3v coloured by chain, side view.
PDB entry 2j3v coloured by chain, top view.
Source organism: Clostridium botulinum
Primary publication:
Structure and action of the binary C2 toxin from Clostridium botulinum.
Schleberger C, Hochmann H, Barth H, Aktories K, Schulz GE
J Mol Biol 364 705-15 (2006)
PMID: 17027031

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130654 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP ribosyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 49.36 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O69275 (Residues: 1-431; Coverage: 100%)
Sequence domains: ADP-ribosyltransferase exoenzyme
Structure domains: Toxin ADP-ribosyltransferase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand GOL 15 x GOL
Ligand SO4 14 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6322
Unit cell:
a: 114.811Å b: 114.811Å c: 162.681Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.188 0.238
Expression system: Escherichia coli BL21

Quick links

Citations

17 review citations

Clostridium difficile binary toxin CDT: mechanism, epidemiology, and potential clinical importance.
Gerding et al. (2014)
16 more

1 mention without citation

Retargeting the Clostridium botulinum C2 toxin to the neuronal cytosol.
Pavlik et al. (2016)