› 2iwe

X-ray diffraction
2.83Å resolution

Structure of a cavity mutant (H117G) of Pseudomonas aeruginosa azurin

Released:
DOI: 10.2210/pdb2iwe/pdb
PDB entry 2iwe coloured by chain, front view.
PDB entry 2iwe coloured by chain, side view.
PDB entry 2iwe coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Click-Chemistry with an Active Site Variant of Azurin
De Jongh TE, Van Roon AMM, Prudencio M, Ubbink M, Canters GW
Eur. J. inorg. Chem. 2006 3861- (2006)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132373 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 128 amino acids
Theoretical weight: 13.88 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand 2IH 2 x 2IH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR78
Spacegroup: P1
Unit cell:
a: 42.664Å b: 49.718Å c: 66.065Å
α: 109.25° β: 94.96° γ: 99.24°
R-values:
R R work R free
0.194 0.192 0.233
Expression system: Escherichia coli

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