› 2iw1

X-ray diffraction
1.5Å resolution

Crystal Structure of WaaG, a glycosyltransferase involved in lipopolysaccharide biosynthesis

Released:
DOI: 10.2210/pdb2iw1/pdb
PDB entry 2iw1 coloured by chain, front view.
PDB entry 2iw1 coloured by chain, side view.
PDB entry 2iw1 coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. W3110
Primary publication:
Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4.
Martinez-Fleites C, Proctor M, Roberts S, Bolam DN, Gilbert HJ, Davies GJ
Chem Biol 13 1143-52 (2006)
PMID: 17113996

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150570 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipopolysaccharide glucosyltransferase WaaG Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 42.34 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25740 (Residues: 1-374; Coverage: 100%)
Gene names: JW3606, b3631, pcsA, rfaG, waaG
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
Ligand U2F 1 x U2F
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 50.718Å b: 88.352Å c: 89.342Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
9 more

13 mentions without citation

Chapter 2: Biogenesis of the cell wall and other glycoconjugates of Mycobacterium tuberculosis.
Kaur et al. (2009)
12 more