› 2irf

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF AN IRF-2/DNA COMPLEX.

Released:
DOI: 10.2210/pdb2irf/pdb
PDB entry 2irf coloured by chain, front view.
PDB entry 2irf coloured by chain, side view.
PDB entry 2irf coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of an IRF-DNA complex reveals novel DNA recognition and cooperative binding to a tandem repeat of core sequences.
Fujii Y, Shimizu T, Kusumoto M, Kyogoku Y, Taniguchi T, Hakoshima T
EMBO J 18 5028-41 (1999)
PMID: 10487755

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115405 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Interferon regulatory factor 2 Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 113 amino acids
Theoretical weight: 13.49 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23906 (Residues: 1-113; Coverage: 32%)
Gene name: Irf2
Sequence domains: Interferon regulatory factor transcription factor
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(P*AP*AP*GP*TP*GP*AP*AP*AP*GP*(5IU)P*GP*A)-3') Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 12 nucleotides
Theoretical weight: 3.87 KDa
Source organism: Mus musculus
Expression system: Not provided
DNA (5'-D(*TP*TP*CP*AP*CP*TP*TP*TP*CP*AP*CP*(5IU)P*T)-3') Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 13 nucleotides
Theoretical weight: 3.98 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand K 6 x K
1 modified residue:
Ligand 5IU 6 x 5IU

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P6522
Unit cell:
a: 132.3Å b: 132.3Å c: 296.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.202 0.243
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

38 review citations

Antiviral actions of interferons.
Samuel CE. (2001)
37 more

9 mentions without citation

Targeted inhibition of STATs and IRFs as a potential treatment strategy in cardiovascular disease.
Szelag et al. (2016)
8 more