› 2iji

X-ray diffraction
2.3Å resolution

Structure of F14H mutant of ColE1 Rom protein

Released:
DOI: 10.2210/pdb2iji/pdb
PDB entry 2iji coloured by chain, front view.
PDB entry 2iji coloured by chain, side view.
PDB entry 2iji coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
New crystal structures of ColE1 Rom and variants resulting from mutation of a surface exposed residue: Implications for RNA-recognition.
Struble EB, Ladner JE, Brabazon DM, Marino JP
Proteins 72 761-8 (2008)
PMID: 18260113

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein rop Chain: A
Molecule details ›
Chain: A
Length: 63 amino acids
Theoretical weight: 7.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03051 (Residues: 1-63; Coverage: 100%)
Gene name: rop
Sequence domains: Rop protein
Structure domains: Helix Hairpins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3121
Unit cell:
a: 39.14Å b: 39.14Å c: 57.442Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.234 0.292
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Influenza virus non-structural protein NS1: interferon antagonism and beyond.
Marc D. (2014)
4 other articles