› 2ief

X-ray diffraction
2.6Å resolution

Structure of the cooperative Excisionase (Xis)-DNA complex reveals a micronucleoprotein filament

Released:
DOI: 10.2210/pdb2ief/pdb
PDB entry 2ief coloured by chain, front view.
PDB entry 2ief coloured by chain, side view.
PDB entry 2ief coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
Structure of the cooperative Xis-DNA complex reveals a micronucleoprotein filament that regulates phage lambda intasome assembly.
Abbani MA, Papagiannis CV, Sam MD, Cascio D, Johnson RC, Clubb RT
Proc Natl Acad Sci U S A 104 2109-14 (2007)
PMID: 17287355

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115396 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Excisionase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 55 amino acids
Theoretical weight: 6.79 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03699 (Residues: 1-55; Coverage: 76%)
Gene name: xis
Sequence domains: Excisionase-like protein
Structure domains: Multidrug-efflux Transporter Regulator; Chain: A; Domain 2
15-mer DNA Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.62 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
19-mer DNA Chain: E
Molecule details ›
Chain: E
Length: 19 nucleotides
Theoretical weight: 5.84 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
34-mer DNA Chain: F
Molecule details ›
Chain: F
Length: 34 nucleotides
Theoretical weight: 10.4 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3121
Unit cell:
a: 111.655Å b: 111.655Å c: 76.268Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.199 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Bacteriophage protein-protein interactions.
Häuser et al. (2012)
3 more

8 mentions without citation

Structure of the cooperative Xis-DNA complex reveals a micronucleoprotein filament that regulates phage lambda intasome assembly.
Abbani et al. (2007)
7 more