› 2i6w

X-ray diffraction
3.1Å resolution

Crystal structure of the multidrug efflux transporter AcrB

Released:
DOI: 10.2210/pdb2i6w/pdb
PDB entry 2i6w coloured by chain, front view.
PDB entry 2i6w coloured by chain, side view.
PDB entry 2i6w coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Crystal structure of the multidrug efflux transporter AcrB at 3.1A resolution reveals the N-terminal region with conserved amino acids.
Das D, Xu QS, Lee JY, Ankoudinova I, Huang C, Lou Y, DeGiovanni A, Kim R, Kim SH
J Struct Biol 158 494-502 (2007)
PMID: 17275331

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux pump subunit AcrB Chain: A
Molecule details ›
Chain: A
Length: 1049 amino acids
Theoretical weight: 113.67 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: R32
Unit cell:
a: 146.3Å b: 146.3Å c: 514.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.305 0.305 0.341

Quick links

Citations

9 review citations

Efflux-mediated drug resistance in bacteria: an update.
Li et al. (2009)
8 more

5 mentions without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
4 more