› 2i4i

X-ray diffraction
2.2Å resolution

Crystal Structure of human DEAD-box RNA helicase DDX3X

Released:
DOI: 10.2210/pdb2i4i/pdb
PDB entry 2i4i coloured by chain, front view.
PDB entry 2i4i coloured by chain, side view.
PDB entry 2i4i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of conserved domains 1 and 2 of the human DEAD-box helicase DDX3X in complex with the mononucleotide AMP.
Högbom M, Collins R, van den Berg S, Jenvert RM, Karlberg T, Kotenyova T, Flores A, Karlsson Hedestam GB, Schiavone LH
J Mol Biol 372 150-9 (2007)
PMID: 17631897

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase DDX3X Chain: A
Molecule details ›
Chain: A
Length: 417 amino acids
Theoretical weight: 47.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00571 (Residues: 168-582; Coverage: 63%)
Gene names: DBX, DDX3, DDX3X
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3121
Unit cell:
a: 67.288Å b: 67.288Å c: 253.47Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.187 0.218
Expression system: Escherichia coli BL21(DE3)

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Citations

25 review citations

Medulloblastomics: the end of the beginning.
Northcott et al. (2012)
24 more

31 mentions without citation

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
30 more