› 2hqg

X-ray diffraction
3.38Å resolution

Conformation of the AcrB Multidrug Efflux Pump in Mutants of the Putative Proton Relay Pathway

Released:
DOI: 10.2210/pdb2hqg/pdb
PDB entry 2hqg coloured by chain, front view.
PDB entry 2hqg coloured by chain, side view.
PDB entry 2hqg coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Conformation of the AcrB multidrug efflux pump in mutants of the putative proton relay pathway.
Su CC, Li M, Gu R, Takatsuka Y, McDermott G, Nikaido H, Yu EW
J Bacteriol 188 7290-6 (2006)
PMID: 17015668

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux pump subunit AcrB Chain: A
Molecule details ›
Chain: A
Length: 1053 amino acids
Theoretical weight: 114.19 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: R32
Unit cell:
a: 144.9Å b: 144.9Å c: 518.635Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.255 0.254 0.275
Expression system: Escherichia coli

Quick links

Citations

30 review citations

Multidrug resistance in bacteria.
Nikaido H. (2009)
29 more

3 mentions without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
2 more