› 2hnt

X-ray diffraction
2.5Å resolution

CRYSTALLOGRAPHIC STRUCTURE OF HUMAN GAMMA-THROMBIN

Released:
DOI: 10.2210/pdb2hnt/pdb
PDB entry 2hnt coloured by chain, front view.
PDB entry 2hnt coloured by chain, side view.
PDB entry 2hnt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallographic structure of human gamma-thrombin.
Rydel TJ, Yin M, Padmanabhan KP, Blankenship DT, Cardin AD, Correa PE, Fenton JW, Tulinsky A
J Biol Chem 269 22000-6 (1994)
PMID: 8071320

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133057 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Thrombin light chain Chain: L
Molecule details ›
Chain: L
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P00734 (Residues: 328-363; Coverage: 6%)
Gene name: F2
Sequence domains: Thrombin light chain
Thrombin heavy chain Chain: C
Molecule details ›
Chain: C
Length: 70 amino acids
Theoretical weight: 8.01 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P00734 (Residues: 364-433; Coverage: 12%)
Gene name: F2
Structure domains: Trypsin-like serine proteases
Thrombin heavy chain Chain: E
Molecule details ›
Chain: E
Length: 81 amino acids
Theoretical weight: 9.39 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P00734 (Residues: 437-517; Coverage: 14%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Thrombin heavy chain Chain: F
Molecule details ›
Chain: F
Length: 105 amino acids
Theoretical weight: 11.97 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P00734 (Residues: 518-622; Coverage: 18%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 126.45Å b: 48.23Å c: 52.43Å
α: 90° β: 96.2° γ: 90°
R-values:
R R work R free
0.156 not available not available
Expression system: Not provided

Quick links

Citations

2 review citations

The protease-activated receptors and their cellular expression and function in blood-related cells.
Hou et al. (1998)
1 more

6 mentions without citation

Taking phototherapeutics from concept to clinical launch.
Vickerman et al. (2021)
5 more