› 2hhe

X-ray diffraction
2.2Å resolution

OXYGEN AFFINITY MODULATION BY THE N-TERMINI OF THE BETA CHAINS IN HUMAN AND BOVINE HEMOGLOBIN

Released:
DOI: 10.2210/pdb2hhe/pdb
PDB entry 2hhe coloured by chain, front view.
PDB entry 2hhe coloured by chain, side view.
PDB entry 2hhe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Chloride ion independence of the Bohr effect in a mutant human hemoglobin beta (V1M+H2deleted).
Fronticelli C, Pechik I, Brinigar WS, Kowalczyk J, Gilliland GL
J Biol Chem 269 23965-9 (1994)
PMID: 7929044

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159519 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemoglobin subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 141 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P69905 (Residues: 2-142; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 145 amino acids
Theoretical weight: 15.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68871 (Residues: 4-147; Coverage: 98%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand HEM 4 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 62.71Å b: 82.38Å c: 53.58Å
α: 90° β: 99.21° γ: 90°
R-values:
R R work R free
0.175 not available not available
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Hemoglobin: Structure, Function and Allostery.
Ahmed et al. (2020)
2 more

1 mention without citation

Dynamics based clustering of globin family members.
Tobi D. (2018)