› 2hf3

X-ray diffraction
1.8Å resolution

Crystal structure of monomeric Actin in the ADP bound state

Released:
DOI: 10.2210/pdb2hf3/pdb
PDB entry 2hf3 coloured by chain, front view.
PDB entry 2hf3 coloured by chain, side view.
PDB entry 2hf3 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structures of expressed non-polymerizable monomeric actin in the ADP and ATP states.
Rould MA, Wan Q, Joel PB, Lowey S, Trybus KM
J Biol Chem 281 31909-19 (2006)
PMID: 16920713

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin-5C Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 41.72 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10987 (Residues: 3-376; Coverage: 100%)
Gene names: Act5C, CG4027
Sequence domains: Actin
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2
Unit cell:
a: 199.7Å b: 54.07Å c: 39.59Å
α: 90° β: 93.16° γ: 90°
R-values:
R R work R free
0.175 0.175 0.213
Expression system: Spodoptera frugiperda

Quick links

Citations

14 review citations

Control of actin filament treadmilling in cell motility.
Bugyi et al. (2010)
13 more

10 mentions without citation

Asymmetric Molecular Architecture of the Human γ-Tubulin Ring Complex.
Wieczorek et al. (2020)
9 more