› 2heg

X-ray diffraction
1.5Å resolution

Phospho-Aspartyl Intermediate Analogue of Apha class B acid phosphatase/phosphotransferase

Released:
DOI: 10.2210/pdb2heg/pdb
PDB entry 2heg coloured by chain, front view.
PDB entry 2heg coloured by chain, side view.
PDB entry 2heg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The First Structure of a Bacterial Class B Acid Phosphatase Reveals Further Structural Heterogeneity Among Phosphatases of Haloacid Deahalogenase Fold
Calderone V, Forleo C, Benvenuti M, Thaller MC, Rossolini GM, Mangani S
J. Mol. Biol. 335 761- (2004)

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Class B acid phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 23.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AE22 (Residues: 27-237; Coverage: 100%)
Gene names: JW4015, aphA, b4055, napA, yjbP
Sequence domains: HAD superfamily, subfamily IIIB (Acid phosphatase)
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand BFD 2 x BFD

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 92.428Å b: 66.631Å c: 90.103Å
α: 90° β: 119.85° γ: 90°
R-values:
R R work R free
0.166 0.164 0.189
Expression system: Escherichia coli BL21(DE3)

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