› 2hc9

X-ray diffraction
1.85Å resolution

Structure of Caenorhabditis elegans leucine aminopeptidase-zinc complex (LAP1)

Released:
DOI: 10.2210/pdb2hc9/pdb
PDB entry 2hc9 coloured by chain, front view.
PDB entry 2hc9 coloured by chain, side view.
PDB entry 2hc9 coloured by chain, top view.
Source organism: Caenorhabditis elegans
Entry authors: Zhan C, Patskovsky Y, Wengerter BC, Ramagopal U, Milstein S, Vidal M, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152708 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine aminopeptidase 1 Chain: A
Molecule details ›
Chain: A
Length: 491 amino acids
Theoretical weight: 52.48 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P34629 (Residues: 1-491; Coverage: 100%)
Gene names: ZK353.6, lap-1
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ZN 2 x ZN
Ligand BCT 1 x BCT
Ligand SO4 2 x SO4
Ligand NA 1 x NA
Ligand GOL 16 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6322
Unit cell:
a: 131.309Å b: 131.309Å c: 126.344Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.15 0.142 0.175
Expression system: Escherichia coli

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