› 2h8q

X-ray diffraction
2Å resolution

Crystal Structure of a Redshifted Mutant (K83M) of the Red Fluorescent Protein dRFP583/dsRed

Released:
DOI: 10.2210/pdb2h8q/pdb
PDB entry 2h8q coloured by chain, front view.
PDB entry 2h8q coloured by chain, side view.
PDB entry 2h8q coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
Novel chromophores and buried charges control color in mFruits.
Shu X, Shaner NC, Yarbrough CA, Tsien RY, Remington SJ
Biochemistry 45 9639-47 (2006)
PMID: 16893165

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein drFP583 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 217 amino acids
Theoretical weight: 25.24 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y8 (Residues: 7-225; Coverage: 97%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRQ 4 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P6522
Unit cell:
a: 92.934Å b: 92.934Å c: 431.593Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.23 0.268
Expression system: Escherichia coli

Quick links

Citations

38 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
37 more

2 mentions without citation

Substrate specificity of thioredoxins and glutaredoxins - towards a functional classification.
Gellert et al. (2019)
1 more