› 2h5a

X-ray diffraction
1.72Å resolution

Complex of the enzyme PMM/PGM with xylose 1-phosphate

Released:
DOI: 10.2210/pdb2h5a/pdb
PDB entry 2h5a coloured by chain, front view.
PDB entry 2h5a coloured by chain, side view.
PDB entry 2h5a coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Complexes of the enzyme phosphomannomutase/phosphoglucomutase with a slow substrate and an inhibitor.
Regni C, Shackelford GS, Beamer LJ
Acta Crystallogr Sect F Struct Biol Cryst Commun 62 722-6 (2006)
PMID: 16880541

Function and Biology Details

Reactions catalysed: 
Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate
Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphomannomutase/phosphoglucomutase Chain: X
Molecule details ›
Chain: X
Length: 463 amino acids
Theoretical weight: 50.46 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26276 (Residues: 1-463; Coverage: 100%)
Gene names: PA5322, algC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand X1P 1 x X1P
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 71.008Å b: 74.551Å c: 86.091Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.213
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Pseudomonas aeruginosa biofilm exopolysaccharides: assembly, function, and degradation.
Gheorghita et al. (2023)
7 other articles

6 mentions without citation

Domain motion and interdomain hot spots in a multidomain enzyme.
Chuang et al. (2010)
5 more