› 2gnv

X-ray diffraction
2.3Å resolution

Crystal structure of non-symbiotic plant hemoglobin from rice, B10 mutant F40L

Released:
DOI: 10.2210/pdb2gnv/pdb
PDB entry 2gnv coloured by chain, front view.
PDB entry 2gnv coloured by chain, side view.
PDB entry 2gnv coloured by chain, top view.
Source organism: Oryza sativa
Primary publication:
Role of phenylalanine B10 in plant nonsymbiotic hemoglobins.
Smagghe BJ, Kundu S, Hoy JA, Halder P, Weiland TR, Savage A, Venugopal A, Goodman M, Premer S, Hargrove MS
Biochemistry 45 9735-45 (2006)
PMID: 16893175

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anaerobic nitrite reductase NSHB1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 165 amino acids
Theoretical weight: 18.3 KDa
Source organism: Oryza sativa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O04986 (Residues: 2-166; Coverage: 99%)
Gene names: GLB1A, HB1, Hb1-1, LOC_Os03g13140, NSHB1, OJ1175C11.5, Os03g0233900, OsJ_10047, Pgb1.1
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand DIO 4 x DIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3121
Unit cell:
a: 125.286Å b: 125.286Å c: 56.25Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.247
Expression system: Escherichia coli BL21

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Citations

6 review citations

The structure and function of plant hemoglobins.
Hoy et al. (2008)
5 more