› 2glq

X-ray diffraction
1.6Å resolution

X-ray structure of human alkaline phosphatase in complex with strontium

Released:
DOI: 10.2210/pdb2glq/pdb
PDB entry 2glq coloured by chain, front view.
PDB entry 2glq coloured by chain, side view.
PDB entry 2glq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural studies of human alkaline phosphatase in complex with strontium: implication for its secondary effect in bones.
Llinas P, Masella M, Stigbrand T, Ménez A, Stura EA, Le Du MH
Protein Sci 15 1691-700 (2006)
PMID: 16815919

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase, placental type Chain: A
Molecule details ›
Chain: A
Length: 484 amino acids
Theoretical weight: 52.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P05187 (Residues: 23-506; Coverage: 94%)
Gene names: ALPP, PLAP
Sequence domains: Alkaline phosphatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

4 bound ligands:
Ligand NAG 2 x NAG
Ligand ZN 2 x ZN
Ligand MG 1 x MG
Ligand SR 1 x SR
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2221
Unit cell:
a: 89.09Å b: 115.299Å c: 107.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.148 0.188

Quick links

Citations

2 review citations

Alkaline phosphatase: an overview.
Sharma et al. (2014)
1 more

5 mentions without citation

Identified the Synergistic Mechanism of Drynariae Rhizoma for Treating Fracture Based on Network Pharmacology.
Lin et al. (2019)
4 more