› 2glo

Solution NMR

Solution structure of the Brinker DNA binding domain in complex with the omb enhancer

Released:
DOI: 10.2210/pdb2glo/pdb
PDB entry 2glo coloured by chain, ensemble of 20 models, front view.
PDB entry 2glo coloured by chain, ensemble of 20 models, side view.
PDB entry 2glo coloured by chain, ensemble of 20 models, top view.
Source organism: Drosophila melanogaster
Primary publication:
DNA recognition by the brinker repressor--an extreme case of coupling between binding and folding.
Cordier F, Hartmann B, Rogowski M, Affolter M, Grzesiek S
J Mol Biol 361 659-72 (2006)
PMID: 16876822

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115305 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Brinker DNA-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 59 amino acids
Theoretical weight: 7.06 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9XTN4 (Residues: 43-101; Coverage: 8%)
Gene names: BRK, Brk, CG9653, Dm brk, Dmel\CG9653, Dmel_CG9653, brinker, brk, brk-RA, ssg-1
Sequence domains: Brinker DNA-binding domain
Structure domains: Homeodomain-like
5'-D(*TP*GP*AP*GP*GP*CP*GP*TP*CP*AP*AP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.69 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
5'-D(*GP*TP*TP*GP*AP*CP*GP*CP*CP*TP*CP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.64 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics; distance geometry
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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Citations

6 review citations

The Decapentaplegic morphogen gradient: from pattern formation to growth regulation.
Affolter et al. (2007)
5 more