› 2gi3

X-ray diffraction
1.8Å resolution

Crystal structure of Glutamyl-tRNA(Gln) amidotransferase subunit A (tm1272) from THERMOTOGA MARITIMA at 1.80 A resolution

Released:
DOI: 10.2210/pdb2gi3/pdb
PDB entry 2gi3 coloured by chain, front view.
PDB entry 2gi3 coloured by chain, side view.
PDB entry 2gi3 coloured by chain, top view.
Source organism: Thermotoga maritima
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194760 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamyl-tRNA(Gln) amidotransferase subunit A Chain: A
Molecule details ›
Chain: A
Length: 476 amino acids
Theoretical weight: 53.26 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X0Z9 (Residues: 1-475; Coverage: 100%)
Gene names: TM_1272, gatA
Sequence domains: Amidase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 3 x ACT
Ligand MPD 3 x MPD
1 modified residue:
Ligand MSE 13 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P3221
Unit cell:
a: 131.776Å b: 131.776Å c: 61.987Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.175 0.201
Expression system: Escherichia coli

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