› 2g8x

X-ray diffraction
1.83Å resolution

Escherichia coli Y209W apoprotein

Released:
DOI: 10.2210/pdb2g8x/pdb
PDB entry 2g8x coloured by chain, front view.
PDB entry 2g8x coloured by chain, side view.
PDB entry 2g8x coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Lee TT, Finer-Moore JS, Stroud RM

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 30.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A884 (Residues: 1-264; Coverage: 100%)
Gene names: JW2795, b2827, thyA
Sequence domains: Thymidylate synthase
Structure domains: Thymidylate synthase/dCMP hydroxymethylase domain

Ligands and Environments

3 bound ligands:
Ligand CO3 3 x CO3
Ligand PO4 2 x PO4
Ligand DTT 2 x DTT
1 modified residue:
Ligand CXM 2 x CXM

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 66.61Å b: 79.69Å c: 92.24Å
α: 90° β: 103.86° γ: 90°
R-values:
R R work R free
0.166 0.164 0.202
Expression system: Escherichia coli

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