› 2g6w

X-ray diffraction
2.14Å resolution

Suicide inhibition of a-Oxamine Synthase: Structures of the Covalent Adducts of 8-Amino-7-oxonanoate Synthase with trifluoroalanine

Released:
DOI: 10.2210/pdb2g6w/pdb
PDB entry 2g6w coloured by chain, front view.
PDB entry 2g6w coloured by chain, side view.
PDB entry 2g6w coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Suicide inhibition of alpha-oxamine synthases: structures of the covalent adducts of 8-amino-7-oxononanoate synthase with trifluoroalanine.
Alexeev D, Baxter RL, Campopiano DJ, Kerbarh O, Sawyer L, Tomczyk N, Watt R, Webster SP
Org Biomol Chem 4 1209-12 (2006)
PMID: 16557306

Function and Biology Details

Reaction catalysed: 
Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO(2) + holo-[acyl-carrier protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
8-amino-7-oxononanoate synthase Chain: A
Molecule details ›
Chain: A
Length: 384 amino acids
Theoretical weight: 41.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P12998 (Residues: 1-384; Coverage: 100%)
Gene names: JW0759, b0776, bioF
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand LLF 1 x LLF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P3112
Unit cell:
a: 58.67Å b: 58.67Å c: 198.97Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.176 0.246
Expression system: Escherichia coli

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Citations

4 review citations

Emerging role of hydrogen sulfide in health and disease: critical appraisal of biomarkers and pharmacological tools.
Whiteman et al. (2011)
3 more