2g2s

X-ray diffraction
1.2Å resolution

Structure of S65G Y66S GFP variant after spontaneous peptide hydrolysis

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-154538 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 6.93 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-65; Coverage: 27%)
Gene name: GFP
Structure domains: Pantoate--beta-alanine Ligase; Chain: A,domain 2
Green fluorescent protein Chain: B
Molecule details ›
Chain: B
Length: 173 amino acids
Theoretical weight: 19.81 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 67-238; Coverage: 72%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 51Å b: 62.3Å c: 68.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
not available not available 0.174
Expression system: Escherichia coli BL21(DE3)