› 2fx8

X-ray diffraction
2.2Å resolution

Crystal structure of hiv-1 neutralizing human fab 4e10 in complex with an aib-induced peptide encompassing the 4e10 epitope on gp41

Released:
DOI: 10.2210/pdb2fx8/pdb
PDB entry 2fx8 coloured by chain, front view.
PDB entry 2fx8 coloured by chain, side view.
PDB entry 2fx8 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of enhanced binding of extended and helically constrained peptide epitopes of the broadly neutralizing HIV-1 antibody 4E10.
Cardoso RM, Brunel FM, Ferguson S, Zwick M, Burton DR, Dawson PE, Wilson IA
J Mol Biol 365 1533-44 (2007)
PMID: 17125793

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209270 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab 4E10 Chains: L, M, N, O
Molecule details ›
Chains: L, M, N, O
Length: 214 amino acids
Theoretical weight: 23.29 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Fab 4E10 Chains: H, I, J, K
Molecule details ›
Chains: H, I, J, K
Length: 227 amino acids
Theoretical weight: 23.86 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Transmembrane protein gp41 Chains: P, Q, R, S
Molecule details ›
Chains: P, Q, R, S
Length: 12 amino acids
Theoretical weight: 1.61 KDa
Source organism: Human immunodeficiency virus type 1 (WMJ2 ISOLATE)
Expression system: Not provided
UniProt:
  • Canonical: P05880 (Residues: 662-673; Coverage: 2%)
Gene name: env

Ligands and Environments

No bound ligands
1 modified residue:
Ligand AIB 4 x AIB

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 53.454Å b: 113.253Å c: 149.961Å
α: 90° β: 94.15° γ: 90°
R-values:
R R work R free
0.235 0.235 0.283
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links

Citations

14 review citations

The immune response during acute HIV-1 infection: clues for vaccine development.
McMichael et al. (2010)
13 more

3 mentions without citation

Structural analysis of B-cell epitopes in antibody:protein complexes.
Kringelum et al. (2013)
2 more