› 2ft7

X-ray diffraction
1.4Å resolution

Structure of Cu(I)azurin at pH 6, with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPM"

Released:
DOI: 10.2210/pdb2ft7/pdb
PDB entry 2ft7 coloured by chain, front view.
PDB entry 2ft7 coloured by chain, side view.
PDB entry 2ft7 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Basic requirements for a metal-binding site in a protein: the influence of loop shortening on the cupredoxin azurin.
Li C, Yanagisawa S, Martins BM, Messerschmidt A, Banfield MJ, Dennison C
Proc Natl Acad Sci U S A 103 7258-63 (2006)
PMID: 16651527

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.58 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 97%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU1 1 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1
Spacegroup: P43212
Unit cell:
a: 51.957Å b: 51.957Å c: 75.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.139 0.178
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Inner- and outer-sphere metal coordination in blue copper proteins.
Warren et al. (2012)
2 more

1 mention without citation

Basic requirements for a metal-binding site in a protein: the influence of loop shortening on the cupredoxin azurin.
Li et al. (2006)