› 2fpl

X-ray diffraction
2.3Å resolution

RadA recombinase in complex with AMP-PNP and low concentration of K+

Released:
DOI: 10.2210/pdb2fpl/pdb
PDB entry 2fpl coloured by chain, front view.
PDB entry 2fpl coloured by chain, side view.
PDB entry 2fpl coloured by chain, top view.
Source organism: Methanococcus voltae
Primary publication:
Crystal structure of Methanococcus voltae RadA in complex with ADP: hydrolysis-induced conformational change.
Qian X, Wu Y, He Y, Luo Y
Biochemistry 44 13753-61 (2005)
PMID: 16229465

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130737 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair and recombination protein RadA Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 35.2 KDa
Source organism: Methanococcus voltae
Expression system: Escherichia coli
UniProt:
  • Canonical: O73948 (Residues: 1-322; Coverage: 100%)
Gene name: radA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand K 3 x K
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P61
Unit cell:
a: 83.454Å b: 83.454Å c: 106.825Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.197 0.249
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Regulation of bacterial RecA protein function.
Cox MM. (2007)
3 more

3 mentions without citation

From Deep Mutational Mapping of Allosteric Protein Landscapes to Deep Learning of Allostery and Hidden Allosteric Sites: Zooming in on "Allosteric Intersection" of Biochemical and Big Data Approaches.
Verkhivker et al. (2023)
2 more